primary human dermal fibroblasts hdfs  (Innoprot Inc)

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Immunofluorescence intensity analysis for COL I, III, IV, V, VI, COL I/COL III ratio, TNMD and aSMA in hTCs and hDFs after 4, 7 and 10 days of culture under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. (∗p < 0.05 vs respective PLLA group at the same time point; ‡p < 0.05 vs respective + TGFB2 group at the same time point; #p < 0.05 vs. respective + MMC group at the same time point; §p < 0.05 between cell types at the same time point and condition; &p < 0.05 vs. respective condition at day 4). N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Immunofluorescence

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Principal component analysis ( A ) of the quantitative proteomic data obtained from hTCs and hDFs after 10 days of culture under TCP, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions, as well as from TT samples. Venn diagram representing the top 100 protein hits in TT group vs. hTCs ( B ) and hDFs ( C ) cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. Gene ontology analysis ( D ) of the top 100 protein hits in TT group vs. hTCs and hDFs cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Gene set enrichment analysis of the quantitative proteomic profile of hDFs cultured for 10 days under PLLA ( A ), +TGFB2 ( B ), +MMC ( C ), and +MMC + TGFB2 ( D ) conditions. All contrasts were performed against hDFs cultured for 10 days under TCP conditions. A gene set containing all protein hits in TT group vs hDFs TCP group (tendon signature) was used for all analyses. Venn diagram summarizing the different core enrichment proteins identified in PLLA, +TGFB2, +MMC and +MMC + TGFB2 groups by means of gene set enrichment analysis ( E ). GO analysis of the core enrichment proteins identified in +MMC + TGFB2 group by means of gene set enrichment analysis ( F ). (NES: normalised enrichment score). N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Heatmap displaying the quantitative matrisome of hTCs and hDFs cultured under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions for 10 days. Hierarchical clustering was applied to all samples. The heatmap displays the z-scores for every matrisome protein identified, being these grouped into the different matrisome categories. Both hTCs and hDFs clustered first in relation to TGFB2 treatment (alone or in combination with MMC), and ultimately in relation with the experimental conditions to which these were subjected. The + MMC + TGFB2 experimental condition exerted the highest changes into the quantitative matrisome of hTCs and hDFs, increasing ECM protein levels throughout all the categories of the matrisome. N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture

Journal: Scientific Reports

Article Title: Cathelicidin LL37-loaded extracellular vesicles from Edwardsiella piscicida promote antibacterial and wound-healing activity

doi: 10.1038/s41598-025-28377-9

Figure Lengend Snippet: In vitro wound-healing activity of Ep EVs-LL37 evaluated using a cell migration assay. LL37, Ep EVs, or Ep EVs-LL37 (5 and 10 µg/mL) were treated to HDF cells and observed the cell migration. ( a ) Representative light microscope images showing the cell-free wound area at different time points. ( b ) Quantification of wound area (%) was performed using ImageJ software. The open wound area (%) was plotted against hours post-treatment (hpt). Data were compared with the negative control (NC) group at each time point and reported as means ± SEM. Statistical analysis was performed using two-way ANOVA followed by Dunnett’s multiple comparison test. (Scale bar = 100 μm; * = p < 0.05. ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001).

Article Snippet: A cell migration assay was carried out using HDF cells (PCS-201-010TM, ATCC, Manassas, VA, USA) to assess the wound-healing effects of Ep EVs-LL37, based on the method described by Edirisinghe et al. .

Techniques: In Vitro, Activity Assay, Cell Migration Assay, Migration, Light Microscopy, Software, Negative Control, Comparison